Translational_Unit

Part:BBa_K2688008:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-08-01)


folC_tu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107


Design Notes

Simplest translational unit design. In our actual design, we added some some spacers for increased expression reliability : 8 bases from +1 of transcription to the RBS beginning, 10 between the CDS and terminator, and 33 after the terminator (before the suffix).


Source

All parts are existing iGEM registry biobrick parts

References